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Sulfo-DIBMA Membrane protein solubilisation & stabilisation

Supplier: CUBE BIOTECH

Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Alpaca Recombinant MSP1D1-His (from E. coli)

Supplier: CUBE BIOTECH

Description: Membrane scaffold protein, alpaca origin.

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfate (CHAPS)

Supplier: CUBE BIOTECH

Description: CHAPS is a zwitterionic detergent and its name is an abbreviation for 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one.

PureCube NHS Activated MagBeads

Supplier: CUBE BIOTECH

Description: PureCube NHS (N-hydroxy succinimide) MagBeads/magnetic beads are your best option to couple biomolecules with free-standing amine groups.

PureCube Cu-NTA MagBeads

Supplier: CUBE BIOTECH

Description: PureCube Cu-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Nonyl-β-D-glucopyranoside ≥99.5%, Crystallization grade

Supplier: CUBE BIOTECH

Description: Nonylglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

N-Undecyl Beta-maltoside ≥99.5%, Crystallization grade

Supplier: CUBE BIOTECH

Description: Mild, non-ionic detergents such as Undecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Synthetic Nanodisc Screening Kit MINI

Catalog Number: (77392-566)

Supplier: CUBE BIOTECH

Description: Synthetic copolymers are capable of solubilizing and stabilizing membrane proteins without the need for detergents. They have the unique capacity to extract the membrane protein of interest while maintaining a near-native environment.

Retrieving Pack

N,N-Dimethyl-1-dodecylamine N-oxide ≥99.5%, Crystallization grade

Supplier: CUBE BIOTECH

Description: LDAO is one of the classic detergents that we offer in crystallization-grade quality at an attractive price. We aliquot our detergents so that they are convenient to use, keep fresh and provide optimal performance.

SMALP Screening Set

Catalog Number: (77392-562)

Supplier: CUBE BIOTECH

Description: Membrane proteins and their composition are as versatile as the whole cell membrane itself. For this reason, it is impossible to predict with 100% accuracy which SMALP product is best suited for the solubilization and subsequent preparation of your membrane protein of interest. We offer this SMALP Screening Kit that consists of our four individual SMAs. This way, you can conveniently screen for the best SMA for your protein before scaling up your assay. In case you would like to extend your polymer screening beyond the reaches of SMA, we offer our Synthetic Nanodisc Screening Kit, which covers both SMA and DIBMA.