PureCube Cu-NTA Agarose

Supplier: CUBE BIOTECH

31410-CU 31403-CU 31405-CU 31412-CU
77393-544EA 5200.7 CAD
77393-544 77393-520 77393-528 77393-536 77393-552
PureCube Cu-NTA Agarose
Protein Purification Protein Purification Reagents

Our PureCube Cu-NTA agarose resins are small agarose resin beads with a diameter of 40 µm.


  • Usage: Specific binding and purification of 6× His-tagged proteins, Binding of Copper-binding proteins
  • Specificity: Affinity to His-tagged proteins, affinity to Copper-binding proteins
  • Binding capacity: >80 mg/ml
  • Bead Ligand: Cu-NTA
  • Bead size: 40 μm
  • Filling quantity: Delivered as a 50% suspension
  • Metal ion capacity: >15 µeqv Cu²⁺/ml
  • pH stability: 2 to 14
  • Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
  • Required equipment:
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 ml tube (min 10000×g)
  • 50 ml centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment


PureCube Cu-NTA agarose resins are used for the purification of active His-tagged proteins from cells and the enrichment of Copper-binding proteins. Our year-long experience in manufacturing agarose resin led to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Cu-NTA suppliers. PureCube Cu-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with great mechanical stability.

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