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Supplier: CUBE BIOTECH

Description: PureCube StrepTactin® agarose resin beads consist of 6% cross-linked agarose coupled to the streptactin protein.

Catalog Number: (77392-868)

Supplier: CUBE BIOTECH

Description: Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria.

Catalog Number: (77392-732)

Supplier: CUBE BIOTECH

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: (77392-564)

Supplier: CUBE BIOTECH

Description: Each membrane protein has its native lipid environment. Therefore, each lipid environment is unique to each membrane protein. Moreover, it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore, one needs a broader selection of polymers since there is not one AASTY that fits each phospholipid composition. To address this question, AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY, which results in different affinities to varying membrane lipid compositions. The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's most excellent features, compared to SMA or DIBMA, is that its nanodiscs are pretty uniform in size. Therefore, the correct fitting nanodisc size must be determined before.

Supplier: CUBE BIOTECH

Description: Octylglucopyranoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

Supplier: CUBE BIOTECH

Description: Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: CUBE BIOTECH

Description: Our PureCube butyl agarose resin is a suspension of purification beads for hydrophobic interaction chromatography.

Catalog Number: (77392-714)

Supplier: CUBE BIOTECH

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: (77392-722)

Supplier: CUBE BIOTECH

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: (77392-704)

Supplier: CUBE BIOTECH

Description: This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

Description: Membrane scaffold protein, rat origin.

Supplier: CUBE BIOTECH

Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-45 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-45 gets it's name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

Description: The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: CUBE BIOTECH

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Catalog Number: (77392-500)

Supplier: CUBE BIOTECH

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Catalog Number: (77392-426)

Supplier: CUBE BIOTECH

Description: Since for each protein, the optimal detergent needs to be screened, and because different detergents can be optimal for extraction, purification, and crystallization steps, Cube Biotech offers detergent screening sets containing small quantities of the most popular detergents at a special discounted price. All our detergents are of high quality, >99% pure, crystallization-grade.