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Supplier: Diagnostic Biosystems

Description: This antibody reacts with cytokeratin 5 intermediate filament protein. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kDa protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas.

Supplier: Diagnostic Biosystems

Description: This antibody is specific to a 45 kDa protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.

Supplier: Diagnostic Biosystems

Description: This antibody is specific to a 40 kDa protein, which is identified as thyroid transcription factor (TTF-1). TTF-1 is expressed in epithelial cells of the thyroid gland and lung. TTF-1 stains primary lung adenocarcinoma and small cell carcinoma but does not stain colon and breast carcinoma.

Supplier: Diagnostic Biosystems

Description: The common acute lymphoblastic leukemia antigen (CALLA/CD10) is a single-pass type II transmembrane metallo-endopeptidase that cleaves and inactivates a variety of peptide growth factors important for signal transduction including the enkephalins, bombesin and substance P. CD10 is expressed by a number of hematopoietic cells such as immature T and B cells, B cells of the germinal centers of lymphoid follicles and granulocytes. It also reacts with a variety of non-hematopoietic cells, including epithelial cells in GI tract and kidney tubular. In liver, the bile canaliculi shows a moderate to strong staining. CD10 has been used for the identification and classification of certain types of malignant lymphoma and leukemia. CD10 is expressed in a high percentage of cases of acute lymphoblastic leukemia, follicular lymphoma, Burkitt's lymphoma, some hematopoietic tumors, and chronic myelogenous leukemias in lymphoid blast crisis. It is also known to be a marker of endometrial stromal cells. It is helpful in differentiating endometrial stromal sarcoma (ESS) from uterine cellular leiomyoma (UCL) and uterine leiomyosarcoma (ULS).

Supplier: Diagnostic Biosystems

Description: This antibody reacts with a 70 kD protein known as Ku (p70).The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86). Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp.

Supplier: Diagnostic Biosystems

Description: Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Catalog Number: (CA76634-622)

Supplier: Diagnostic Biosystems

Description: This antibody reacts with chains of human IgG. Nonspecific antibodies have been removed by solid-phase adsorption.

Catalog Number: (CA76634-618)

Supplier: Diagnostic Biosystems

Description: This antibody does not show any cross-reactivity with kappa light chain. It stains B cell follicles in human lymphoid tissues. The mantle zone shows a mosaic pattern of labeling while the germinal centers show a coarse meshwork pattern of staining.

Supplier: Diagnostic Biosystems

Description: This antibody reacts with human factor VIII related antigen. It stains endothelial cells and megakaryocytes in various human tissues. This antibody does not cross-react with any other cell types.

Catalog Number: (CA76634-852)

Supplier: Diagnostic Biosystems

Description: This antibody recognizes CD5, which is a transmembrane protein, is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5(+) while the follicle center cell lymphoma is CD5(-).

Catalog Number: (CA76634-846)

Supplier: Diagnostic Biosystems

Description: The clone F7.2.38 is specific for the non-glycosylated epsilon chain of the human CD3 molecule. Clone F7.2.38 recognizes T cells in the thymus, bone marrow, peripheral lymphoid tissue and blood and is a pan T cell marker. The CD3 molecule consists of five different polypeptide chains with molecular weights ranging from 16 to 28 kDa. The CD3 antigen is first detected in early thymocytes, and its appearance probably represents one of the earliest signs of commitment to the T cell lineage.

Supplier: Diagnostic Biosystems

Description: This antibody recognizes human retinoblastoma gene protein. Rb gene, a prototype of tumor suppressor genes, has been associated with development and/or progression of bladder cancer as well as sarcoma and small cell lung cancer. Functional Rb gene loss has been reported to occur in bladder cancer and osteosarcoma. A significantly poorer tumor-free survival rate was noted for those patients who had a tumor with an altered Rb protein with or without vascular invasion.

Supplier: Diagnostic Biosystems

Description: This antibody reacts specifically with B cells at the pre B cell stage. It stains a majority of acute leukemias of precursor B cell type, B cell lymphomas and some myelomas. The mb-1 polypeptide along with B29 polypeptide forms an antigen receptor complex on the B cell, performing signal transduction and phosphorylation functions.

Supplier: Diagnostic Biosystems

Description: This antibody is specific to a Sialoglycoprotein of 27 to 32 kDa known as CD99 or MIC2 gene product. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes and granulosa cells of the ovary, Sertoli cells of testis, pancreatic islet cells and some endothelial cells. Mature granulocytes express very little or no CD99.

Supplier: Diagnostic Biosystems

Description: This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to anti-estrogen therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm.

Supplier: Diagnostic Biosystems

Description: This antibody recognizes a single chain glycoprotein of 105/120 kDa, identified as CD30/Ki-1. Its epitope is located between aa112-412. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120 kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).