You Searched For: CUBE BIOTECH

Catalog Number: (77392-708)

Supplier: CUBE BIOTECH

Description: This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP2N2 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Catalog Number: (77392-698)

Supplier: CUBE BIOTECH

Description: This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Catalog Number: (77392-710)

Supplier: CUBE BIOTECH

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Catalog Number: (77392-870)

Supplier: CUBE BIOTECH

Description: Sensory rhodopsin-2 (SR-2) is a 7-transmembrane helice protein that acts as a phototaxis receptor in archaebacteria.

Supplier: CUBE BIOTECH

Description: Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

Description: Membrane scaffold protein, rat origin.

Supplier: CUBE BIOTECH

Description: Mild, non-ionic detergents such as Undecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Supplier: CUBE BIOTECH

Description: LDAO is one of the classic detergents that we offer in crystallization-grade quality at an attractive price. We aliquot our detergents so that they are convenient to use, keep fresh and provide optimal performance.

Supplier: CUBE BIOTECH

Description: With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Supplier: CUBE BIOTECH

Description: Nonylglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

Catalog Number: (77392-500)

Supplier: CUBE BIOTECH

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Catalog Number: (77392-426)

Supplier: CUBE BIOTECH

Description: Since for each protein, the optimal detergent needs to be screened, and because different detergents can be optimal for extraction, purification, and crystallization steps, Cube Biotech offers detergent screening sets containing small quantities of the most popular detergents at a special discounted price. All our detergents are of high quality, >99% pure, crystallization-grade.

Catalog Number: (77392-766)

Supplier: CUBE BIOTECH

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: CUBE BIOTECH

Description: Membrane scaffold protein, alpaca origin.

Supplier: CUBE BIOTECH

Description: CHAPS is a zwitterionic detergent and its name is an abbreviation for 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one.