Human Recombinant MSP1D1-D73C (from E. coli)
Supplier: CUBE BIOTECH
Membrane scaffold protein, human origin.
- Origin: Human
- Extinction coefficient (in water) ε280: 25440 M⁻¹cm⁻¹
- Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
- Affinity tag: N-Terminal His-tag
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after removing the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 D73C creates nanodiscs of 9 to 10 nm in diameter. This product contains an additional point mutation, D73C. This additional cysteine can be used to modify the MSP protein further and, thus, the nanodisc. An example of such a modification would be the addition of a Biotin-tag.
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