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Supplier: Bachem Americas
Description: Sequence: Sulfo-N-succinimidyl 4-maleimidobutyrate sodium salt
Synonym(s): Sulfo-GMBS sodium salt

Supplier: CUBE BIOTECH
Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: VWR
Description: A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection.
Supplier: VWR International
Description: SureLight® 488 NHS Ester is a bright green fluorescent dye with excitation suited to the 488nm laser line, fluorescent microscopy and flow cytometry. It offers a much more stable signal generation in imaging and flow cytometry than FITC and is half the cost of Alexa Fluor® 488 with no royalties or licensing fees.

SDS

Catalog Number: (89139-648)
Supplier: Biotium
Description: is a water-soluble B-complex vitamin. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.


Supplier: CUBE BIOTECH
Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: Enzo Life Sciences

Supplier: Spectrum Chemical Mfg. Corp.
Description: Biotin, Powder, USP is a water-soluble B vitamin. 
Catalog Number: (CAPI29129)
Supplier: Thermo Scientific
Description: Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes.

Supplier: TCI America
Description: CAS Number: 58-85-5
MDL Number: MFCD00005541
Molecular Formula: C10H16N2O3S
Molecular Weight: 244.31
Purity/Analysis Method: >98.0% (T)
Form: Crystal
Melting point (°C): 230
Specific rotation [a]20/D: 90.5 deg (C=2, 0.1mol/L NaOH sol.)
Catalog Number: (CAPI21111)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-KMUS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (16.3 angstroms).

Catalog Number: (CAPI22324)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-GMBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).

Supplier: Thermo Scientific Chemicals
Description: Essential vitamin that is important for amino acid and energy metabolism
Catalog Number: (103003-306)
Supplier: Anaspec Inc
Description: Green fluorescent biotin used for studying avidin binding


Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).
Catalog Number: (CAPI22327)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.

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