Sulfo-SMA Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)
Usage: Membrane protein solubilization & stabilization
Full-Name: Poly(styrene-co-N′,N′-dimethyl(maleimidoethyl)ammonium propane sulfonate) copolymer
Formula Weight: 8,000 g/mol ± 1000 g/mol
Buffer: 50 mM HEPES
pH upon addition of dH2O: 7.5 ± 0.3
Total pH stability: 4-10
State: Lyophilized Powder
Recommended concentration range upon use: 1% to 5%
Solubility: > 10 % in H2O
Mg2+ Tolerance: over 100 mM
Ca2+ Tolerance: over 100 mM
Color: White to slightly yellow
Caution: Shipping Temperature: Ambient temperature. Storage of lyophilized copolymer: -20°C for several years. Storage of dissolved copolymer: 2-8°C for several day.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...