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Supplier: Thermo Scientific
Description: DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.
Supplier: Thermo Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.
Supplier: Thermo Scientific
Description: Use to efficiently transfer protein from an electrophoresis gel to a membrane in northern, Southern, and Western blotting applications.

Catalog Number: (CAPI84786)
Supplier: Thermo Scientific
Description: Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) are a ready-to-use mix of recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems.

Catalog Number: (CAPI22329)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

SDS


Catalog Number: (CAPI22106)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Catalog Number: (CAPI22360)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Catalog Number: (CAPI22330)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce BMH is a long-arm, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

Catalog Number: (CAPI22349)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Supplier: Thermo Scientific
Description: Thermo Scientific SuperSignal West Pico Complete IgG Detection Kit is a a kit containing goat anti-mouse or anti-rabbit secondary antibody HRP conjugate for the detection of mouse or rabbit primary antibody
Catalog Number: (CAPI36978)
Supplier: Thermo Scientific
Description: Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

Supplier: Thermo Scientific
Description: Ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme.
Supplier: Thermo Scientific
Description: SuperSignal® ELISA Femto maximum sensitivity substrate utilizes the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications.
Catalog Number: (CAPI20227)
Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules

Catalog Number: (CAPI20266)
Supplier: Thermo Scientific
Description: Thermo Scientific CarboxyLink Coupling Resin is for covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Catalog Number: (CAPI20163)
Supplier: Thermo Scientific
Description: The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

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