EMCH.TFA (E-Maleimidocaproic acid hydrazine trifluoroacetic acid salt) ≥90% (by NMR), Pierce™

Supplier: Thermo Scientific

Synonyms: EMCH, E-Maleimidocaproic acid hydrazine trifluoroacetic acid salt, 3,3'-N-[ε-Maleimidocaproic acid] hydrazide trifluoroacetic acid salt, EMCH.TFA, 6-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanehydrazide 2,2,2-trifluoroacetate, 6-Maleimidohexanehydrazide trifluoroacetate, 6-Maleimidocaproic acid hydrazide TFA, N-ε-Maleimidocaproic acid hydrazide trifluoroacetic acid salt

22106
CAPI22106EA 635.48 CAD
CAPI22106
EMCH.TFA (E-Maleimidocaproic acid hydrazine trifluoroacetic acid salt) ≥90% (by NMR), Pierce™
EMCH.TFA (E-Maleimidocaproic acid hydrazine trifluoroacetic acid salt)

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).


  • Reactive groups: maleimide and hydrazide
  • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
  • Mid-length (11.8Å), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups


3,3'-N-[ε-Maleimidocaproic acid] hydrazide, trifluoroacetic acid salt (EMCH) is a water-soluble, heterobifunctional, membrane permeable crosslinker. It contains a sulfhydryl reactive maleimide group and carbonyl reactive hydrazide group at each end of a 6-carbon spacer arm. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thiol ether bonds, along with release of the maleimide leaving group. Proteins with cysteine residues not involved in disulfide bond formation are targets for maleimide reactive groups. Carbonyl groups, whilst not found naturally on proteins can be formed by ring sugar reduction to form aldehydes which react with hydrazides at pH5.5-7.5.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₀H₁₅N₃O₃·C₂HF₃O₂
Melting Pt: 99…110°C
Storage Temperature: Refrigerator
MDL Number: MFCD07357647
CAS Number: 151038-94-7

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Specification Test Results

Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Amine, Hydrazide, Maleimide
Spacer Mid-Length
Form Solid
Quantity 50 mg
Type Crosslinker
Solubility Water


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