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You Searched For: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-10147-298

Radiance Plus Chemiluminescent Substrate, Azure Biosystems

Catalog Number: (10147-298)
Supplier: Azure Biosystems
Description: Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

SDS Product available on GSA Advantage®


Product Image-10147-340

Fluorescent Wash Buffer, Azure Biosystems

Catalog Number: (10147-340)
Supplier: Azure Biosystems
Description: Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

SDS Product available on GSA Advantage®


Product Image-10147-348

Transfer Buffer, Azure Biosystems

Catalog Number: (10147-348)
Supplier: Azure Biosystems
Description: Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

SDS Product available on GSA Advantage®


Product Image-10147-296

Radiance Q Chemiluminescent Substrate, Azure Biosystems

Catalog Number: (10147-296)
Supplier: Azure Biosystems
Description: Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

SDS Product available on GSA Advantage®


Product Image-10147-336

Protein Free Blot Blocking Buffer, Azure Biosystems

Catalog Number: (10147-336)
Supplier: Azure Biosystems
Description: Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

SDS Product available on GSA Advantage®


Product Image-CA10746-470

Amersham ECL Western Blotting Start Detection Reagents, Cytiva

Supplier: Cytiva
Description: Chemiluminescence detection reagent for detection of high to medium abundant proteins.

SDS Certificates

Product Image-CA76492-478

Fluorescent Labeling Reagents, CypHer5E NHS Ester, Cytiva

Supplier: Cytiva
Description: CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Image Unavailable-CA95040-078L

ECL Western Blotting ECF Substrate, Cytiva

Catalog Number: (CA95040-078L)
Supplier: Cytiva
Description: Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

SDS Certificates


Product Image-CA95038-280L

Amersham ECL Gold Hybridization Buffer, Cytiva

Catalog Number: (CA95038-280L)
Supplier: Cytiva
Description: ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

SDS


Product Image-CA89238-012

Western blotting detection reagent, Amersham™ ECL™ Prime

Supplier: Cytiva
Description: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

SDS Certificates

Product Image-CA95016-840L

Pharmalytes® IEF Carrier Ampholytes, Cytiva

Supplier: Cytiva
Description: Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

SDS Certificates

Product Image-CA89128-838

Cy™ Amidites

Supplier: Cytiva
Description: Cy™ fluorescent amidites for incorporation of Cy3, Cy3.5, Cy5, Cy5.5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Product Image-CA97068-824

Amersham ECL Western Blotting Select Detection Reagent, Cytiva

Catalog Number: (CA97068-824)
Supplier: Cytiva
Description: Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

SDS Certificates


Image Unavailable-89141-658

Acridinium Ester, (Trigger Solution), Enzo Life Sciences

Catalog Number: (89141-658)
Supplier: Enzo Life Sciences
Description: Species Independent
Product Image-CA95040-068L

Amersham™ ECL™ Prime Blocking Reagent

Catalog Number: (CA95040-068L)
Supplier: Cytiva
Description: Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

SDS Certificates


Product Image-CA95056-442L

DeStreak Rehydration Reagent, Cytiva

Supplier: Cytiva
Description: The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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