Catalog Number: (CA200061-462)

Supplier: Enzo Life Sciences

Description: The exotoxin produced by B. anthracis is composed of three proteins: a cell-receptor binding protein known as Protective Antigen (PA), and two enzymes, Edema Factor (EF) and Lethal Factor (LF). An 83 kDa form of PA binds to a cell surface receptor and, upon proteolytic activation to a 63 kDa fragment, heptamerizes to form a membrane channel that mediates the entry of three molecules of LF or EF into the cells. PA and LF form Lethal Toxin, which is the dominant virulence factor produced by B. Anthracis and is the major cause of death in infected animals.

Catalog Number: (CA200061-474)

Supplier: Enzo Life Sciences

Description: Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.

Catalog Number: (CA200061-484)

Supplier: Enzo Life Sciences

Description: Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adaptor proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.

Catalog Number: (CA95045-822)

Supplier: Enzo Life Sciences

Description: Src is a member of the tyrosine kinase family that was originally identified as the oncogene of Rous sarcoma virus. The catalytic domain contains a tyrosine autophosphorylation site that increases kinase activity when phosphorylated. The Src family of tyrosine kinase is involved in a variety of receptor-induced signal transduction pathways such as immune recognition rec

SDS

Catalog Number: (CA95045-198)

Supplier: Enzo Life Sciences

Description: This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

Supplier: Enzo Life Sciences

Description: Assay diluent and lysis reagent for cyclic nucleotides.

Catalog Number: (CA95043-882)

Supplier: Enzo Life Sciences

Description: Neutralizing buffer for low pH solutions, for use with cyclic nucleotide and isoprostane kits.

Catalog Number: (CA101106-274)

Supplier: Enzo Life Sciences

Description: HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.

Catalog Number: (CA101106-516)

Supplier: Enzo Life Sciences

Description: 15-deoxy-Δ12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate Δ12-PGJ2. 15-d-PGJ2 is physiologically present in body fluids at concentration of 10-12 to 10-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ2 exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the γ isoform of the peroxisome proliferators-activated receptor (PPAR-γ). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-γ-independent mechanism mediated by the generation of reactive oxygen species (ROS).

Supplier: Enzo Life Sciences

Description: TCP-1alpha is a 60 kDa subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of Actin and Tubulin. This protein is a member of the Chaperonin family, which includes E. coli GroEL, the mitochondrial Heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein, and the archaebacterial protein TF55. These Chaperonins assist the folding of proteins upon ATP hydrolysis.

Catalog Number: (CA95052-784)

Supplier: Enzo Life Sciences

Description: Plate (Clear), Enzo Life Sciences

Supplier: Enzo Life Sciences

Description: The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

Supplier: Enzo Life Sciences

Description: Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

SDS

Supplier: Enzo Life Sciences

Description: FKBP59, also known as Hsp56/p59 is an immunophilin that is a component of Hsp70/Hsp90/steroid receptor complexes. Expression of FKBP59 is increased in response to heat shock and ischemic stress, and is thought to play a role in the folding and cytoplasmic/nuclear distribution of steroid receptors.

Catalog Number: (CA200061-460)

Supplier: Enzo Life Sciences

Description: The GST superfamily of enzymes play an important role in detoxification of xenobiotics by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. There are four main GST classes; alpha, mu, pi, and tau. GST-P is a member of the pi class of GSTs, and its expression is specifically increased in the early stages of rat hepatocarcinogenesis. Due to its high affinity for glutathione, GST is frequently utilized as an approximately 26 kDa fusion tag for the expression and purification of recombinant proteins.

Supplier: Enzo Life Sciences

Description: Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins at the neuromuscular junction during development. Agrin, MuSK, and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation.