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siRNA/miRNA transfection reagent - INTERFERin®

INTERFERin® is a powerful, ready to use siRNA and miRNA reagent which provides more than 90% silencing efficiency at 1 nM siRNA in a wide variety of cells.

  • Great silencing using as little as 1 nM siRNA
  • Over 90% gene silencing in a wide variety of cells
  • Suitable for transfection miRNA and other oligonucleotides
  • Gentle mode of action for more robust data and excellent cell viability
  • Compatible with serum and antibiotics

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Description

Several publications show that transfecting low siRNA concentrations avoids off-target effects. Indeed, these unwanted non specific side-effects are observed when transfecting siRNA at high concentrations. Hence, the reliability of the experimental data can be increased by using as little siRNA as possible. This is why INTERFERin® has been specifically designed to provide high silencing efficiency using low siRNA concentrations.

INTERFERin®-mediated delivery of 1 nM of a specific siRNA shows selective and highly efficient knockdown of gene expression, while a competitor (L2K) needs at least 10 nM siRNA to reach 50% silencing efficiency.

INTERFERin ® only requires 1 nM siRNA for efficient gene silencing. 3LL cells stably expressing firefly luciferase were transfected with an anti-Luc siRNA using INTERFERin ® or competitor L2K according to the manufacturer’s recommendation. Luciferase expression was measured after 48 h using a conventional assay. No inhibition was observed with control siRNA.

For many adherent cell lines or primary cells, 1 nM siRNA is sufficient to obtain more than 90 % gene silencing. For suspension cell lines, 80 % silencing can still be reached by INTERFERin® using 5 nM siRNA.

Successfully transfected cell lines and silencing efficiencies obtained with INTERFERin®.

The cationic components of INTERFERin® require very little oligonucleotide amount, resulting in a gentle transfection process. In addition, the easy and robust protocol ensures reproducible results. Oligonucleotides like miRNA can be transfected with small quantities of INTERFERin® resulting in strong gene silencing or stimulation of gene expression. Whatever the concentration of INTERFERin® used, the effect stays approximately the same making INTERFERin® an economical reagent.

INTERFERin® is already efficient at very low amounts. PC-3 cells were seeded in a 6-well plate and transfected with miR-373 (10 nM) using 4, 6 or 8 µl per well of INTERFERin®. 72h post-transfection, E-cadherin expression was analyzed by Western Blot. The assay was normalized using β-actin expression. The quantification was expressed by taking the control situation as reference.

Examples of citations

Carduner, L., Picot, C. R., Leroy-Dudal, J., Blay, L., Kellouche, S., Carreiras, F. (2014). Cell cycle arrest or survival signaling through alphav integrins, activation of PKC and ERK1/2 lead to anoikis resistance of ovarian cancer spheroids., Exp Cell Res 320, 329-4.

Hu, B., Castillo, E., Harewood, L., Ostano, P., Reymond, A., Dummer, R., Raffoul, W., Hoetzenecker, W., Hofbauer, G. F., Dotto, G. P. (2012).Multifocal epithelial tumors and field cancerization from loss of mesenchymal CSL signaling., Cell 149, 1207.

Lai, L., Song, Y., Liu, Y., Chen, Q., Han, Q., Chen, W., Pan, T., Zhang, Y., Cao, X., Wang, Q. (2013). MicroRNA-92a negatively regulates Toll-like receptor (TLR)-triggered inflammatory response in macrophages by targeting MKK4 kinase., J Biol Chem 288, 7956.

Testimonials

“I use your INTERFERin siRNA reagent in primary human Macrophages with terrific results.”
Jason Hammonds, Emory University, United States

“I prefer INTERFERin [vs two competitors]. Awesome product with very competitive price.”
Latonia Taliaferro-Smith, Winship Cancer Institute of Emory University, United States"

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