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Supplier: Thermo Scientific
Description: An advanced formula for removing high-affinity bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce Protein G and Protein G Plus Agarose is a premium-quality affinity resin for antibody purification.
Supplier: Thermo Scientific
Description: Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
Catalog Number: (CAPI20266)
Supplier: Thermo Scientific
Description: Thermo Scientific CarboxyLink Coupling Resin is for covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Supplier: Thermo Scientific
Description: Thermo Scientific HisPur Ni-NTA Magnetic Beads are high-capacity nickel-IMAC beads for affinity purification of His-tagged fusion proteins in manual or automated formats.
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through <i>in vivo</i> metabolic labeling and chemoselective ligation.
Supplier: Thermo Scientific
Description: Pierce™ Anti-HA magnetic beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
Supplier: Thermo Scientific
Description: Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.
Catalog Number: (CAPI78840)
Supplier: Thermo Scientific
Description: Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.

Catalog Number: (CAPI80370)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.

Catalog Number: (CAPI36978)
Supplier: Thermo Scientific
Description: Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

Supplier: Thermo Scientific
Description: SuperSignal® ELISA Femto maximum sensitivity substrate utilizes the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications.
Catalog Number: (CAPI22360)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Catalog Number: (CAPI23011)
Supplier: Thermo Scientific
Description: Pierce™ MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.

Catalog Number: (CAPI22663)
Supplier: Thermo Scientific
Description: 36460762

Catalog Number: (CAPI22349)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

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