Pierce™ Restore™ PLUS Western Blot Stripping Buffer, Thermo Scientific
Supplier: Thermo Scientific
An advanced formula for removing high-affinity bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
- Ready and easy to use - no dilution necessary, no offensive odours and room temperature storage
- Compatible with commonly used Western blotting reagents and other materials - use on nitrocellulose and PVDF membranes, stored wet or dry; works with blocking buffer, enzyme conjugate and chemiluminescent substrate of choice
- Cost effective - saves valuable time and sample; strip blots effectively the first time
- Robust, but gentle - transferred proteins remain viable; strip the same blot up to five times
- Flexible - strip and re-probe to optimize antibody concentrations; strip and re-probe for new antigen of interest
As an alternative formulation of the original Restore Western Blot stripping buffer, Restore PLUS stripping buffer is designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C (71.6°F) with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.
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