You Searched For: 2,4,6-Trimethylthiophenol

Catalog Number: (103008-678)

Supplier: Anaspec Inc

Description: This is an amidated peptide derived from residues 361-393 of human p53 tumor suppressor protein with acetylation at Lys382. This peptide is biotin-labeled at its N-terminus with a 6-carbon LC linker. Activated p53 functions to induce cell cycle arrest and apoptosis in response to signals such as DNA damage. Acetylation of p53 reduces ubiquitination by MDM2 and increases p53 half-life in vivo.
Sequence:Biotin-LC-GSRAHSSHLKSKKGQSTSRHK-K(Ac)-LMFKTEGPDSD-NH2
MW:4034.7 Da
% peak area by HPLC:95
Storage condition:-20° C

Catalog Number: (103008-518)

Supplier: Anaspec Inc

Description: This peptide is derived from amino acid residues 372-389 of human p53 tumor suppressor protein and is acetylated at Lys382. It is biotinylated at its N-terminus through a 6-carbon LC linker. Activated p53 functions to induce cell cycle arrest and apoptosis in response to signals such as DNA damage. Acetylation of p53 reduces ubiquitination by MDM2 and increases p53 half-life in vivo.
Sequence:Biotin-LC-KKGQSTSRHK-K(Ac)-LMFKTEG
MW:2473 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Thermo Scientific

Description: Photoactivatable Biotin, Biotin-LC-ASA, Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups.

SDS Certificates

Catalog Number: (103010-372)

Supplier: Anaspec Inc

Description: Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester, Biotin, NHS ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

Supplier: G-Biosciences

Description: The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

SDS

Catalog Number: (102996-878)

Supplier: Anaspec Inc

Description: The native peptide AAKIQASFRGHMARKK (27173) is a synthetic PKC substrate derived from the sequence of neurogranin, a naturally occurring PKC substrate. The peptide is proven to be a PKC substrate with quite good specificity. It is phosphorylated by purified PKC with a Km of 150 nM. No significant phosphorylation of the peptide by either PKA or by CaMK 2 is reported. Substituting Arg36 with Ile causes a significant reduction in the affinity for PKC. Replacing Lys30 with Arg enhances the catalytic efficiency (Vmax/Km) for PKC but diminishes the selectivity of the substrate for PKC. It is generally considered that basic amino acids on both sides of the phosphorylated Ser are important structural determinants in PKC substrates. In addition, the presence of particular basic amino acids (Arg vs Lys) may also contribute to the degree of selectivity of a substrate for PKC.
Sequence:Biotin-LC-AAKIQASFRGHMARKK
MW:2139.6 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: G-Biosciences

Description: The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Supplier: G-Biosciences

Description: The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Catalog Number: (103007-704)

Supplier: Anaspec Inc

Description: This is beta-amyloid (10-35) with a Lys added on the C-terminus, biotin is attached to the side chain of this Lys.
Sequence: YEVHHQKLVFFAEDVGSNKGAIIGLM-K(Biotin)-NH2
Molecular Weight: 3256.9 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: G-Biosciences

Description: The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation

Supplier: Thermo Scientific

Description: This antibody labeling system uses nickel chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions

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Supplier: CUBE BIOTECH

Description: Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: Bachem Americas

Description: Sequence: Sulfo-N-succinimidyl 4-maleimidobutyrate sodium salt
Synonym(s): Sulfo-GMBS sodium salt

Supplier: CUBE BIOTECH

Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Catalog Number: (CA82022-802)

Supplier: G-Biosciences

Description: N-(ε-Maleimidocaproyloxy) sulfo succinimide ester Heterobifunctional Cross Linker.

Supplier: Thermo Scientific

Description: Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

SDS Certificates