You Searched For: Western Blot Detection Systems

Supplier: G-Biosciences

Description: G-Biosciences' SWIFT™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes

SDS

Catalog Number: (CA76102-544)

Supplier: Cytiva

Description: The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

SDS Certificates

Supplier: Cytiva

Description: Amersham Hybond LFP 0.2 PVDF is a low-fluorescent, hydrophobic membrane, with 0.2 µm pore size, for protein transfer. Amersham Hybond LFP is optimized for fluorescent detection in Western blotting applications and for use with the Amersham ECL Plex detection system.

Certificates

Catalog Number: (CA95038-570L)

Supplier: Cytiva

Description: Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

SDS Certificates

Catalog Number: (76545-500)

Supplier: BIOASSAY SYSTEMS

Description: Direct assays of peroxidases, ELISA, immunochromogenic chemistry, western blot.

Supplier: Cytiva

Description: Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.

SDS Certificates

Supplier: Thermo Scientific Chemicals

Description: For chemiluminecent detection of HRP-based Western blotting systems.

Certificates

Catalog Number: (RL100-401-B21)

Supplier: Rockland Immunochemical

Description: . This Antibody Has Been Tested For Use In Elisa And Western Blotting Can Be Used To Detect Blt (14-Residue) In Recombinant Expression Systems. Blt Is The Minimal Substrate Requirement Of Bira-Catalyzed Biotinylation.

Supplier: Cytiva

Description: Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

SDS Certificates

Supplier: Columbia Biosciences

Description: Streptavidin Conjugates, Columbia Biosciences

SDS

Supplier: G-Biosciences

Description: G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Supplier: Cytiva

Description: Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.

SDS Certificates

Supplier: G-Biosciences

Description: The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

SDS

Catalog Number: (76235-518)

Supplier: Rockland Immunochemical

Description: AKT1 Western Bloting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Catalog Number: (76235-516)

Supplier: Rockland Immunochemical

Description: AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Catalog Number: (76235-514)

Supplier: Rockland Immunochemical

Description: AKT2 Western Bloting kit allows for the detection of endogenous protein levels of human AKT2 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT2. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT2 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT2 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.