Luminol and oxidizing solutions (each bottle)

Supplier: Thermo Scientific Chemicals

J61462-AP J61462-AK J61462-AK J61462-AP

CAAAJ61462-APEA 535.41 CAD

AAJ61462-AK CAAAJ61462-AP CAAAJ61462-AK AAJ61462-AP

Luminol and oxidizing solutions (each bottle)

Luminol and oxidizing solutions

For chemiluminecent detection of HRP-based Western blotting systems.

Applications: Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes, as it reacts with iron found in hemoglobin. It is used by biologists in cellular assays for the detection of copper, iron, and cyanides, as well as of specific proteins by western blot. For chemiluminecent HRP-based detection of western blotting systems. Microestimation of glucose and glucose oxidase using enzyme-induced chemiluminescence6,7 - Inhibitor of poly ADP Ribose Synthase8 - Demonstrations of the phenomenon of chemiluminescence, using different sensitizers to produce different colors of light.

Notes: Mix equal amounts of luminol and oxidizing solution, then incubate the membrane for 1 min. Expose membrane to x-ray film for at least one minute. Adjust exposure as necessary.

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