You Searched For: Western Blot Detection Systems

Catalog Number: (76235-516)

Supplier: Rockland Immunochemical

Description: AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Catalog Number: (CAPIMA1020D488)

Supplier: Thermo Scientific

Description: This Antibody has been successfully used in Western blot with human and mouse samples. Western blot analysis of MA1-014-HRP specifically detects SOX2 protein at ~36 kDa in the nucleus of embryonal carcinoma cells. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Supplier: G-Biosciences

Description: G-Biosciences' femtoCHROMO™- AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Catalog Number: (10101-320)

Supplier: Prosci

Description: FERD3L is an evolutionarily conserved bHLH transcription factor that is identified in fly, mouse and man. Nervous system expression of this protein is detected in fly and mouse.

Catalog Number: (10101-318)

Supplier: Prosci

Description: FERD3L is an evolutionarily conserved bHLH transcription factor that is identified in fly, mouse and man. Nervous system expression of this protein is detected in fly and mouse.

Catalog Number: (10086-044)

Supplier: Proteintech

Description: DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)

Catalog Number: (CARL200401139S)

Supplier: Rockland Immunochemical

Description: Anti-Trypsin Antibody is suitable for western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Catalog Number: (CA10486-474)

Supplier: Analytik Jena CA

Description: For use with Analytik Jena US Advanced Imaging Systems.

Catalog Number: (10086-240)

Supplier: Proteintech

Description: The M13 family has seven members, including ECEL1 (endothelin-converting enzyme-like 1), one of the newest members. ECEL1 is expressed predominantly in the central nervous system. It has been proposed that the enzyme has a role in the nervous regulation of the respiratory system.It may contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.It has 2 isoforms produced by alternative splicing and 3 glycosylation sites.It can be detected a band of 95kDa in CHO cells by western blot because of the heavily glycosylated.

Catalog Number: (75953-722)

Supplier: Biotium

Description: In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Supplier: G-Biosciences

Description: picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase

SDS

Supplier: Biotium

Description: In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Supplier: Biotium

Description: In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Catalog Number: (CAPI37527)

Supplier: Thermo Scientific

Description: This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Catalog Number: (76098-588)

Supplier: Bioss

Description: This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests.

Catalog Number: (76098-586)

Supplier: Bioss

Description: This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests.