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Tricyclic amide linker resin (DL-form) 200-400 mesh

Supplier: Bachem Americas
Description: Ramage resin is highly suitable for the preparation of peptide amides by the Fmoc-strategy and is recommended for C-terminal Phe, Tyr and Ile. Cleavage has been performed with 50 % TFA in CH₂Cl₂ or 95% aqueous TFA. Scavengers may be required. Tricyclic amide linker resin has been used for the synthesis of fluorogenic substrates for continuous assay of aminopeptidase P. Bachem additionally offers the Ramage amide linker (Q-2745) to be coupled to other types of carrier resin.

Image Unavailable-D-2040.0005BA

Xanthenyl linker resin 200-400 mesh

Supplier: Bachem Americas
Description: Sieber amide resin has been developed for the Fmoc-SPPS of fully t-butyl protected peptide amides. Cleavage can be achieved with 1% TFA in methylene chloride. Scavengers may be required. As N-methylation increases the acid-lability of the peptide bond, Malakoutikhah et al. employed Sieber resin for obtaining short peptide amides containing multiple MePhe residues.

Image Unavailable-D-2125.0001BA

PAL (Peptide Amide Linker) resin 200-400 mesh

Catalog Number: (D-2125.0001BA)
Supplier: Bachem Americas
Description: Useful for the synthesis of peptide amides using Fmoc strategy. Cleavage has been performed with 50 % TFA in CH₂Cl₂ or 95% aqueous TFA. Scavengers may be required.
Image Unavailable-D-1675.0005BA

Rink amide AM resin 200-400 mesh

Supplier: Bachem Americas
Description: Fmoc-Rink amide-AM resin, a standard resin for the Fmoc-SPPS of peptide amides. Cleavage was performed with 50 % TFA in CH₂Cl₂ or 95% aqueous TFA. Scavengers may be required. Bachem additionally offers the Rink amide (RAM) linker (Q-1660).

Image Unavailable-CA95029-294

EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) crosslinker

Supplier: G-Biosciences
Description: EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

Image Unavailable-TCD2672-5G

4-[(2,4-Dimethoxyphenyl)-(fmoc-amino)methyl]phenoxyacetic acid ≥98.0% (by HPLC, titration analysis)

Catalog Number: (TCD2672-5G)
Supplier: TCI America
Description: CAS Number: 126828-35-1 MDL Number: MFCD00153509 Molecular Formula: C32H29NO7 Molecular Weight: 539.58 Purity/Analysis Method: <gt/>98.0% (HPLC,T) Form: Crystal Melting point (°C): 180 Storage Temperature: 0-10°C

SDS Certificates


Image Unavailable-103008-716

Histone H4 (1-23)-GGK, Biotin

Catalog Number: (103008-716)
Supplier: Anaspec Inc
Description: This peptide is of Histone H4 (1-23) biotinylated through a GGK linker on the C-terminus. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Sequence:SGRGKGGKGLGKGGAKRHRKVLR-GGK(Biotin)-NH2
MW:2828.4 Da
% peak area by HPLC:95
Storage condition:-20° C
Image Unavailable-CAPI20593

DSG (Disuccinimidyl glutarate), Pierce™

Catalog Number: (CAPI20593)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Certificates


Product Image-CAPI77149

EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

Supplier: Thermo Scientific
Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

SDS Certificates

Product Image-CAPI22360

SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate), Pierce™

Catalog Number: (CAPI22360)
Supplier: Thermo Scientific
Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

SDS Certificates


Image Unavailable-TCA0541-025G

DL-Asparagine monohydrate ≥99.0% (by titrimetric analysis)

Supplier: TCI America
Description: CAS Number: 3130-87-8
MDL Number: MFCD00151039
Molecular Formula: C4H8N2O3
Molecular Weight: 132.12
Purity/Analysis Method: >99.0% (T)
Form: Crystal

Certificates

Product Image-CA95017-030

NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Catalog Number: (CA95017-030)
Supplier: Cytiva
Description: NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

SDS Certificates


Image Unavailable-103008-678

Human [Lys(Ac)382]-p53 (361-393), Biotin

Catalog Number: (103008-678)
Supplier: Anaspec Inc
Description: This is an amidated peptide derived from residues 361-393 of human p53 tumor suppressor protein with acetylation at Lys382. This peptide is biotin-labeled at its N-terminus with a 6-carbon LC linker. Activated p53 functions to induce cell cycle arrest and apoptosis in response to signals such as DNA damage. Acetylation of p53 reduces ubiquitination by MDM2 and increases p53 half-life in vivo.
Sequence:Biotin-LC-GSRAHSSHLKSKKGQSTSRHK-K(Ac)-LMFKTEGPDSD-NH2
MW:4034.7 Da
% peak area by HPLC:95
Storage condition:-20° C
Product Image-97061-340

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Supplier: VWR
Description: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

SDS Certificates

Image Unavailable-D-2570.0001BA

4-Formyl-3-methoxyphenyloxymethyl on polystyrene resin 200-400 mesh

Supplier: Bachem Americas
Description: For Fmoc solid phase synthesis of N-alkyl peptide amides by reductive amination.

Image Unavailable-D-2080.0025BA

4-(2',4'-Dimethoxyphenyl-fmoc-aminomethyl)phenoxymethyl on polystyrene 0.80-1.20 mmol/g 200-400 mesh

Supplier: Bachem Americas
Description: Resin for the Fmoc-SPPS of peptide amides. Cleavage was achieved with 50 % TFA in CH₂Cl₂. or 95% TFA Scavengers may be required. (EP 0 285 562 B1 licensed to Bachem)

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