Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Supplier: VWR

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Synonyms: DL-1,4-Dithiothreitol , DTT , cis-2,3-Dihydroxy-1,4-butanedithiol , (R*,R*)-1,4-Dimercapto-2,3-butandiol

VWRV0281-25G VWRV0281-5G

97061-340EA 231.14 CAD

97061-340 97061-338

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Dithiothreitol (DTT, Cleland's reagent)

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Formula: C₄H₁₀O₂S₂
MW: 154.25 g/mol
Boiling Pt: ∼125 °C (1013 hPa)
Melting Pt: 42 C…43 °C
Flash Pt: >110 °C

MDL Number: MFCD00004877
CAS Number: 3483-12-3

https://ca.vwr.com/stibo/web/std.lang.all/98/19/2299819.jpg

Specification Test Results

Abs.@283nm (0.02M, Water)	= 0.05
DNase	none detected
Loss on Drying	0.5 %
Melting Point	39 °C - 43 °C
Oxidized DTT	0.5 %
Protease	none detected
Purity (-SH Content)	99.4 %
RNase	none detected
Solubility (5%, Water)	pass

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