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SensoLyte® 520 Enterokinase Activity Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-626)
Supplier: Anaspec Inc
Description: Enterokinase is a heterodimeric serine protease produced by cells in the duodenal wall
Image Unavailable-103003-134

Crosstide Peptide, FAM (Carboxyfluorescein)

Supplier: Anaspec Inc
Description: Crosstide is a peptide analog of glycogen synthase kinase-3 (GSK-3) that functions as a natural substrate for Akt/PKB. It displays similar specificities towards PKBα, PKBβ and PKBγ isoforms. It is also recognized by MAPKAP kinase-1 and p70S6K. The fluorescent and biotinlyated peptides demonstrate similar enzyme activities.

Image Unavailable-89139-260

5-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator

Catalog Number: (89139-260)
Supplier: Biotium
Description: 5-Carboxyfluorescein has a pKa of 6.5 and can be used as a pH sensor. The excitation spectrum of the dye responds to pH similarly to that of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
Image Unavailable-103005-942

520 MMP FRET Substrate XIV, QXL™ 520-FAM, AnaSpec

Catalog Number: (103005-942)
Supplier: Anaspec Inc
Description: Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is a sensitive substrate for assaying MMP-1, 2, 3, 7, 8, 9, 12, and 13 activities, Abs/Em = 494/521 nm.
Sequence:QXL™ 520 -γ-Abu-P-Cha-Abu-Smc-HA-Dab(5-FAM)-AK-NH2 (Smc=S-Methyl-L-cysteine)
MW:1913.1 Da
% peak area by HPLC:95
Storage condition:-20° C
Image Unavailable-103006-568

Cls Substrate

Catalog Number: (103006-568)
Supplier: Anaspec Inc
Description: This peptide is a second complement component (C2), the physiological substrate for the proenzyme Cls, first complement component. The complement system is a central component of host defense but can also contribute to the inflammation seen in pathological conditions. The C1s protease of the C1 complex initiates the host defense pathway. This peptide employs 5FAM/QXL520 FRET pair for quantitation of complement enzyme activity.
Sequence:5-FAM-SLGRKIQIK(QXL 520)-NH2
MW:2019.2 Da
% peak area by HPLC:95
Storage condition:-20° C
Image Unavailable-89139-718

Reactive Dye Conjugates, Biotium

Supplier: Biotium
Description: Reactive dyes containing an amine functional group.

Product Image-470320-302

Orbit Atoms, Bonds and Accessories

Catalog Number: (470320-302)
Supplier: Cochranes of Oxford
Description: Order extra atoms, bonds, and accessories to supplement your Minit® Sets.
Image Unavailable-103007-076

Renin 520 Peptide

Catalog Number: (103007-076)
Supplier: Anaspec Inc
Description: This FRET peptide is a specific substrate for renin. This renin peptide substrate may be used for screening of renin inhibitors. In the FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This substrate is employed in the SensoLyte® 520 Renin Assay Kit, cat # AS-72040 from AnaSpec.
MW: 2000 - 2200 Da
%Peak area by HPLC:≥95%
Storage condition: -20°C
Image Unavailable-103007-238

Proapoptotic Peptide

Catalog Number: (103007-238)
Supplier: Anaspec Inc
Description: This is the pro-apoptotic peptide composed of D-amino acids, 5-FAM labeled. This alpha-helical amphipathic peptide is toxic to eukaryotic cells if internalized by a suitable targeting mechanism. It disrupts mitochondrial membranes upon receptor-mediated cell internalization and causes programmed cell death.
Sequence:5-FAM-klaklakklaklak-NH2
MW:1881.3 Da
% peak area by HPLC:95
Storage condition:-20° C
Image Unavailable-CAAAAL13439-MD

5(6)-Carboxyfluorescein mixture of isomers 97%

Supplier: Thermo Scientific Chemicals
Description: 5(6)-Carboxyfluorescein mixture of isomers 97%

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Image Unavailable-103010-624

SensoLyte® 520 Factor Xa Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-624)
Supplier: Anaspec Inc
Description: Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits
Image Unavailable-89139-248

5(6)-Carboxyfluorescein ≥95% (by HPLC) fluorescent pH indicator

Catalog Number: (89139-248)
Supplier: Biotium
Description: 5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
Image Unavailable-89139-262

6-Carboxyfluorescein ≥98% (by HPLC) fluorescent pH indicator

Catalog Number: (89139-262)
Supplier: Biotium
Description: 6-Carboxyfluorescein has a pKa of 6.5 and can be used as a pH sensor. The excitation spectrum of the dye responds to pH similarly to that of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
Image Unavailable-103010-664

SensoLyte® Plus 520 MMP-2 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.

Catalog Number: (103010-664)
Supplier: Anaspec Inc
Description: The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.
Image Unavailable-103010-186

SensoLyte® 520 Aggrecanase-1 Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-186)
Supplier: Anaspec Inc
Description: Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases
Image Unavailable-103005-938

520 MMP FRET Substrate XII, QXL™ 520-FAM, AnaSpec

Catalog Number: (103005-938)
Supplier: Anaspec Inc
Description: Matrix Metalloproteinases (MMPs) are a large family of endopeptidases. Collectively, MMPs can degrade all kinds of extracellular matrix proteins, and can also process a number of bioactive molecules. They are known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands, and chemokine/cytokine inactivation. MMPs are also thought to play a major role in cell behaviors such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, and host defense.
This peptide is a sensitive substrate for assaying MMP-1, 2, 7, 8, 12 and 13 activities, Abs/Em = 494/521nm.
Sequence:5-FAM-RPKPYA-Nva-WM-K(QXL™ 520)-NH2
MW:2125.4 Da
% peak area by HPLC:95
Storage condition:-20° C
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