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Protein Purification Methods: A Comprehensive Overview The basic aim in protein purification is to isolate one particular protein of interest from other contaminating proteins to study its structure and function. This page will provide an overview...

Overview of Affinity Chromatography Affinity chromatography involves the binding affinity between the target protein and a ligand immobilized on a stationary phase within a chromatography column. One of the advantages of affinity chromatography is...

Proteomics Tools and Equipment Protein Expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms utilized for protein expression must be easy to culture and maintain grow rap...

Protein Expression Technology Kits and Resources Protein expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms used for protein expression technology must be easy to cult...

Proteomics PROTEIN EXPRESSION ☐ Protein Expression Mammalian Routine Tightly Controlled Ultra-Pure ☐ Prokaryotic Cells Competent Cells Electroporation Induction Reagents PET Vectors for Gateway Cloning ☐ Transfection Insect Cells Mammalian Cells ☐...

What is western blotting? Overview of western blotting Western blotting is the technique for identifying proteins of interest in a sample using gel electrophoresis to separate sample proteins. This technique has paved the way in biomedical science...

PowerPoint Presentation Comparative Proteomics Kit I: Protein Profiler Module Protein Profiler Kit Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville CA Kirk Brown Lead Instructor Edward Teller E...

Overview of Affinity Chromatography Affinity chromatography involves the binding affinity between the target protein and a ligand immobilized on a stationary phase within a chromatography column. One of the advantages of affinity chromatography is...

Protein Sample Preparation for Mass Spectrometry Protein sample preparation In order to run a successful mass spectrometry analysis you will need to properly prepare your sample. This includes a number of steps that may be specialized depending on...

Protein Electrophoresis and Western Blotting Getting accurate and high-quality results depends on having the supplies and equipment you need to do your job efficiently and effectively. That’s why Avantor® carries your preferred products from brand...

Protein Electrophoresis and Western Blotting Getting accurate and high-quality results depends on having the supplies and equipment you need to do your job efficiently and effectively. That’s why Avantor® carries your preferred products from brand...

Protein Sample Preparation for Mass Spectrometry Protein sample preparation In order to run a successful mass spectrometry analysis you will need to properly prepare your sample. This includes a number of steps that may be specialized depending on...

Chemiluminescent Westerns are popular assays for assessing protein expression. In this indirect detection method chemiluminescent substrates emit light when reacted with an antibody conjugated to an enzyme. The emitted light is captured and archiv...

Detecting in-gel fluorescence enables the analysis of protein gels immediately after electrophoresis without staining or Western blotting. Separation of proteins by electrophoresis is a cornerstone of protein analysis. After separation identificat...

Chemiluminescent Westerns Chemiluminescent Western blotting is an indirect method for detecting proteins bound on a membrane. The method relies on an enzyme-substrate reaction that emits light which is traditionally detected on x-ray film. Chemilu...

Introduction Stain-Free gel technology enables detection of protein bands in gels and on transfer membranes without using colorimetric or fluorescent stains. Stain-Free gels (Bio-Rad) contain a trihalocompound within the gel matrix that produces a...