Protein Expression Technology, Kits, and Resources
Protein expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms used for protein expression technology must be easy to culture and maintain, grow rapidly, and produce large amounts of protein.
Application Notes
Literature
Webinars
Featured Suppliers
Hardy Diagnostics: We are your partner in Microbiological testing
From environmental control in cleanrooms to pathogen detection in Food & Beverage, Cosmetics & Personal Care and Cannabis labs, Hardy provides solutions.
Cells Choose Seradigm
Seradigm’s unique collection and manufacturing techniques preserve more of the naturally-occurring growth factors in sera that cells require to thrive.
Construct Generation and Introduction
Successfully introduce and incorporate genetic material into your target cell or tissue with effective protein expression technology from Avantor.
Grow - Prokaryotic Cells (Yeast & Bacterial Cell Culture)
Find the right protein expression products to help ensure the maximum yield of prokaryotic cells.
Grow - Eukaryotic cells (Mammalian & Insect Cell Culture)
Avantor is your source for everything your lab needs to help ensure high-volume eukaryotic cell growth.
QA/QC
Quality control is a critical step in protein expression. Shop Avantor for reliable quality assurance supplies.
Featured Products
Hardy Trio.Bas™
Hardy Diagnostics introduces the next generation of microbial air samplers for viable particles.
Cytiva™ HyrTryp™ Cell dissociation reagent
Dissociate and harvest cells confidently with HyrTryp™ cell dissociation reagent, an animal-derived component-free alternative to porcine trypsin.
Beckman Vi-CELL
Validated reagent with appropriate cleaning and disinfectant solutions give you peace of mind. Don't put your experiments at risk and go with reagents you can trust.
Hardy Lok-Tight™ Contact Plates
An added measure to ensure sterility in your environmental testing program.
Hardy Fluid A
Hardy's approach to manufacturing ensures all sterility media and rinse fluids provide the highest level of clarity for improved reading accuracy, thus significantly reducing the risk of incorrect interpretation and false results.
Avantor® Seradigm FBS
Avantor Seradigm Select Grade FBS provides superior performance and consistency in cost-sensitive applications.
What Is a Protein Expression System?
A protein expression system is a genetic construct designed to produce a protein. Protein expression systems are used in research and are also used to produce antibodies, vaccines, and enzymes commercially.
What Is the Difference Between Protein Expression and Protein Purification?
The difference between protein expression and protein purification is that protein expression is the process of producing proteins while protein purification is the process of isolating proteins of interest.
The level of purity needed corresponds to the end use of the protein. A purity of 90-95% may qualify a protein for research purposes, but proteins meant for use in pharmaceutical applications require purity levels up to 99.99%.
The purification workflow follows several steps:
- Protein is extracted from cells by lysing.
- A centrifuge separates soluble from insoluble components.
- Chromatography purifies the protein.
- Protein is quantified and checked for purity.
What Is the Purpose of Protein Expression?
The purpose of protein expression is to manufacture a functional protein in a laboratory that can be used for research or commercial use. Protein expression technology may be accomplished using protein expression kits designed for specific types of expression.
Various cell types can be used for protein expression, including:
- Bacteria
- Yeast
- Insect
- Mammalian
Protein expression can also be done by using a cell-free protein expression system.
Large-scale recombinant protein expression is used to produce enzymes, antibodies, or vaccines. This type of protein production requires a high volume, and the system must make it easy to culture, maintain, and grow proteins rapidly.
Shop the Best Products for Protein Expression Technology
We carry innovative protein expression products from trusted suppliers at Avantor. Shop a wide range of high-performance lab equipment, tools and supplies for protein expression systems.
Avantor also delivers the best values in the marketplace. Look through our portfolio of exclusive brands or work with us for a tailored approach. Avantor can help you set science in motion with our additional services.