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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-CA101228-190

T4 RNA Ligase 1 (ssRNA Ligase), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Product Image-CA101228-192

E. coli DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Product Image-CA102715-910

T4 RNA Ligase 2 Truncated, KQ, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Product Image-CA103218-906

NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs

Catalog Number: (CA103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly
Image Unavailable-CA80031-486

pT7Blue Perfectly Blunt® Cloning Kit, Novagen®, EMD Millipore

Catalog Number: (CA80031-486)
Supplier: MilliporeSigma
Description: The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
Product Image-CA200064-420

9°N™ DNA Ligase, New England Biolabs

Catalog Number: (CA200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA
Product Image-CA102877-802

NEBUILDER HiFi DNA Assembly® Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

Certificates

Product Image-CA102715-942

ELECTROLIGASE®, New England Biolabs

Catalog Number: (CA102715-942)
Supplier: New England Biolabs (NEB)
Description: ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.
Product Image-CA101228-182

T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Certificates

Product Image-CA102877-808

NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Catalog Number: (CA102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E
Product Image-CA102715-946

Instant Sticky-end Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Product Image-CA103307-256

RtcB Ligase

Catalog Number: (CA103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in <i>E. coli</i>.
Product Image-CA102969-104

NEBNext Ultra II Ligation Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Product Image-CA101417-962

T4 RNA Ligase 2 Truncated, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Product Image-CA76299-644

NEB Cloning Competent E. coli Sampler, New England Biolabs

Catalog Number: (CA76299-644)
Supplier: New England Biolabs (NEB)
Description: The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.
Product Image-CA76407-348

Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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