You Searched For: Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.


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Catalog Number: (BDH7830-1)
Supplier: VWR International
Description: N.I.S.T traceable to SRM 919.

Supplier: VWR
Description: VWR® TRIS EDTA Buffer, Biotechnology Grade
Catalog Number: (97062-834)
Supplier: VWR
Description: VWR® Sodium Acetate, 3M Solution, pH 5.2, Biotechnology Grade

Catalog Number: (97062-836)
Supplier: VWR
Description: VWR® EDTA 0.5M, Biotechnology Grade

Supplier: VWR
Description: VWR® EDTA 0.5M, Biotechnology Grade
Catalog Number: (10128-588)
Supplier: QUALITY BIOLOGICAL, INC.
Description: Low-EDTA 1X, pH 8.0 is molecular biology grade TE buffer used to store DNA and RNA. EDTA chelates Mg2+ and other divalent metals ions - (inhibits DNAse and RNAse to suppress DNA and RNA degradation).

Supplier: QUALITY BIOLOGICAL, INC.
Description: TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.

Small Business Enterprise Minority or Woman-Owned Business Enterprise

Supplier: QUALITY BIOLOGICAL, INC.
Description: EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

Small Business Enterprise Minority or Woman-Owned Business Enterprise

Supplier: GROWCELLS
Description: Tris (tris[hydroxymethyl]aminoethane) is widely used as a component of buffer solutions

Supplier: GROWCELLS
Description: Nuclease free TE is used to solubilize DNA/RNA while providing protection from degradation

Catalog Number: (10128-582)
Supplier: QUALITY BIOLOGICAL, INC.
Description: TNE Buffer is often used as a base material to generate lysis buffers.

Small Business Enterprise Minority or Woman-Owned Business Enterprise


Catalog Number: (CA-EM3700)
Supplier: MilliporeSigma
Description: DNA Extraction Buffer

Catalog Number: (CAMB-016)
Supplier: Rockland Immunochemical
Description: 3.0 M Sodium Acetate, pH 5.2

SDS


Catalog Number: (CA101227-462)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity


Catalog Number: (CA101227-398)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes


Catalog Number: (CA101227-460)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity


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