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BCECF AM ester ≥95% (by HPLC) fluorescent pH indicator

Supplier: Biotium
Description: BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.

Image Unavailable-89139-238

BCECF AM ester 1 mg/mL in anhydrous DMSO ≥95% (by TLC) fluorescent pH indicator

Catalog Number: (89139-238)
Supplier: Biotium
Description: BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.
Image Unavailable-CA80050-710

BCECF AM ester ≥85% (by HPLC), Calbiochem®, Sigma-Aldrich®

Catalog Number: (CA80050-710)
Supplier: MilliporeSigma
Description: A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubule

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Product Image-89165-974

BCECF AM ester ≥95% (by HPLC), Ultrapure

Catalog Number: (89165-974)
Supplier: Enzo Life Sciences
Description: pH indicator
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Furaptra (Mag-Fura-2) AM Ester ≥95% (by TLC) fluorescent indicator, for calcium determination

Supplier: Biotium
Description: Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation

Image Unavailable-10018-200

Rhod-590 AM ester fluorescent indicator, for calcium determination

Catalog Number: (10018-200)
Supplier: Biotium
Description: Membrane-permeant form of Rhod-590 calcium indicator dye that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. Rhod-590 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Rhod-590 by endogenous esterases once it enters cells.
Image Unavailable-89139-184

FLUO 3/AM 1 mM in anhydrous DMSO fluorescent indicator, for calcium determination

Catalog Number: (89139-184)
Supplier: Biotium
Description: Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.
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Indo-1 AM ≥96% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium
Description: Indo-1, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate cell loading. Indo-1-AM Ester itself does not bind Ca2+ , but it is readily hydrolyzed to indo-1 by endogenous esterases once it enters cells.

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Fura 2-AM ≥98% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium
Description: Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.

Image Unavailable-89139-180

FLUO 3/AM ≥95% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium
Description: Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

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BAPTA-AM ≥98% (by TLC) ion chelator, for calcium determination

Supplier: Biotium
Description: BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

Image Unavailable-89139-198

Fura 2-AM 1 mM in anhydrous DMSO fluorescent indicator, for calcium determination

Catalog Number: (89139-198)
Supplier: Biotium
Description: Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.
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5,5’-Dimethyl BAPTA AM ester ≥95% (by TLC) ion chelator, for calcium determination

Catalog Number: (89139-168)
Supplier: Biotium
Description: Dimethyl BAPTA AM ester is a membrane permeable form of Dimethyl BAPTA that can be loaded into cells by incubation. Dimethyl BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Dimethyl BAPTA by cytoplasmic esterases.
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5,5'-difluoro BAPTA AM ester ≥95% (by TLC) ion chelator, for calcium determination

Catalog Number: (89139-164)
Supplier: Biotium
Description: Difluoro BAPTA AM ester is a membrane permeable form of Difluoro BAPTA that can be loaded into cells by incubation. Difluoro BAPTA AM ester itself does not bind calcium, but once inside the cell is converted into Difluoro BAPTA by cytoplasmic esterases. See #50006 for information on Difluoro BAPTA.
Product Image-89139-240

BCECF (2′,7′ –bis-(Carboxyethyl)-5-(and-6)-carboxyfluorescein) ≥95% (by HPLC) fluorescent pH indicator

Catalog Number: (89139-240)
Supplier: Biotium
Description: BCECF (2′,7′ –bis-(Carboxyethyl)-5-(and-6)-carboxyfluorescein) is the most widely used fluorescent pH sensor. Because its pKa (6.97) is close to physiological pH, BCECF can detect cytosolic pH change with high sensitivity.
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Calcein AM ≥90% (by HPLC) fluorescent dye, for histology

Supplier: Biotium
Description: Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

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