Generating CRISPR cell models, an overview, challenges and latest technologies
CRISPR/Cas9 is a revolutionary technology to irreversibly and accurately modify the genome. To successfully perform genome editing it is essential to understand which techniques are available to deliver CRISPR/Cas9 components into cells. As transfection experts, Polyplus-transfection gives an overview of how CRISPR/Cas9 achieves precise genome editing, delves into key challenges and considerations that come with delivery of CRISPR/Cas9 components into cells, and presents latest technologies to address these challenges. The webinar will finish with a short Q&A session, with the opportunity for follow-up discussions.
Polyplus-transfection offers a large range of transfection reagents that cover available CRISPR approaches:
- Transfection of plasmid DNA encoding Cas9, gRNA and gene of interest (jetPRIME and jetOPTIMUS)
- Transfection of gRNA and mRNA encoding Cas9 (jetMESSENGER)
- Delivery of RNP, meaning the Cas9 protein complexed with the gRNA (jetCRISPR and SpCas9 nuclease)
Presented by: Alengo Nyamay'antu, PhD
Alengo Nyamay’antu is Scientific Support and Communication Specialist at Polyplus-transfection® SA, a leading biotechnology company that provides solutions for delivery of nucleic acids in research, bioproduction and therapeutics. Alengo completed her MSc in structural and functional biochemistry at the University of Lyon and went on to specialise in protein biochemistry at the University of Manchester. She then continued to develop and widen her scientific and communication skills by joining the Max Planck Institute for Biomedicine as a postdoctoral researcher. Alengo is particularly keen on exchanging and discussing science, and looks forward to the upcoming webinar to get you started on genome editing with the latest technologies.