You Searched For: protein purification system

Catalog Number: (10087-698)

Supplier: Proteintech

Description: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

Supplier: Thermo Scientific

Description: The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

SDS Certificates

Catalog Number: (76118-330)

Supplier: Bioss

Description: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Catalog Number: (10094-068)

Supplier: Proteintech

Description: S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.

Catalog Number: (10097-298)

Supplier: Proteintech

Description: Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation.

Catalog Number: (CA101414-366)

Supplier: Omega Bio-Tek

Description: The E.Z.N.A.® plasmid systems are able to isolate plasmid DNA by means of HiBind™ columns, Mag-Bind™ beads, or isopropanol precipitation

Catalog Number: (CA95056-086L)

Supplier: Cytiva

Description: HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

SDS Certificates

Catalog Number: (76119-680)

Supplier: Bioss

Description: Probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. May play a role in protein processing in limb, cardiac and reproductive system development. May play a role in cytokinesis.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Description: StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible.

Catalog Number: (10087-458)

Supplier: Proteintech

Description: GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein. Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Catalog Number: (10090-022)

Supplier: Proteintech

Description: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Supplier: Thermo Scientific

Description: Formulation: Polypropylene (2mL capacity); Attached snap-caps Sufficient For: Sample collection 72 tubes

Catalog Number: (10082-554)

Supplier: Proteintech

Description: The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Catalog Number: (10087-678)

Supplier: Proteintech

Description: GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Catalog Number: (10094-946)

Supplier: Proteintech

Description: Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD1 is one Sm components of the small nuclear ribonucleoprotein complexes(snRNPs). It's a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions throuth nonspecific electrostatic contacts with RNA. This antibody detectes 13-16 kDa (SMD1) and 30 kDa (SMD1/SMD2 dimer form; PMID:15525645).

Catalog Number: (10087-662)

Supplier: Proteintech

Description: GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.