You Searched For: viral+rna

Catalog Number: (10325-088)

Supplier: Bioss

Description: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence.

Supplier: IBI Scientific

Description: I-Blue Midi Plasmid Endotoxin-Free Kits optimize isolation of plasmid DNA from E.coli with a simple lysis, bind, wash, and elute protocol. The kits include I-Blue lysis buffer, which helps prevent common handling errors, ensuring efficient cell lysis and SDS precipitation.

Catalog Number: (CA95017-499L)

Supplier: Cytiva

Description: illustra™ RNAspin 96 kit is a flexible, high-throughput solution for total RNA isolation.

SDS

Catalog Number: (76108-220)

Supplier: Bioss

Description: Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.

Supplier: Cytiva

Description: For rapid purification of labelled DNA (≥20 bases) from unincorporated labelled nucleotides in a volume of 25 to 50 μl using spin column chromatography.

SDS Certificates

Supplier: Biotium

Description: AccuBlue™ and AccuClear™ dsDNA quantitation assays provide sensitive, accurate and easy-to-use dsDNA quantitation assays for fluorescent microplate readers in 96-well or 384-well formats.

Supplier: IBI Scientific

Description: The miRNA Isolation kits are designed for the purification of microRNA (miRNA) and other small cellular RNAs from tissue samples and cultured cells

Catalog Number: (CA95016-898L)

Supplier: Cytiva

Description: Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.

SDS

Supplier: Omega Bio-Tek

Description: Magnetic separation device-A consists of 24 powerful magnetic rods that fit between the wells of commercially-available 96-well microplates

Certificates

Catalog Number: (77020-282)

Supplier: Zymo Research

Description: The 96-Well 2.0 ml deep well plate can be used in automated liquid handlers for the high-throughput purification of DNA and/or RNA.

Supplier: Cytiva

Description: The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

SDS Certificates

Catalog Number: (CA76193-690)

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Description: Optical Strip Cap, for use with the Mx3000P & Mx3005P AriaMx real time QPCR Systems, Also for use with the 2200 TapeStation system, Size: 60 strip caps

Supplier: MilliporeSigma

Description: Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids.

Certificates

Catalog Number: (CA76538-636)

Supplier: Cytiva

Description: Sera-Xtracta™ HMW DNA Kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.

Supplier: Omega Bio-Tek

Description: Isolate DNA from molluscs and insects using spin columns.

Catalog Number: (10412-824)

Supplier: Bioss

Description: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.