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Supplier: Thermo Scientific Chemicals

Description: MDL: MFCD00007056 Beilstein Registry No.: 398632 Fieser: 1,321 2,175 6,233

Certificates

Supplier: Corning

Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilizes a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimized buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Certificates

Supplier: Corning

Description: AxyPrep™ Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post sequencing reaction

SDS Certificates

Catalog Number: (CA82022-502)

Supplier: G-Biosciences

Description: SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyzes the carboxy peptide bond of Arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilize enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.

Catalog Number: (CA103218-922)

Supplier: New England Biolabs (NEB)

Description: In nature, <i>S. pyogenes</i> Cas9 is programmed with two separate RNAs, the crRNA and tracrRNA.

Catalog Number: (CA76469-804)

Supplier: Cytiva

Description: AutoScreen 96-well plate is used for purification of sequencing samples reactions prior to analysis on capillary array electrophoresis (CAE) sequencers such as MegaBACE 1000 or ABI Prism sequencing instruments.

SDS

Catalog Number: (CA12001-812)

Supplier: Lonza

Description: Long Ranger™ sequencing reagents are designed for the high performance sequencing on ABI Prism® 377 and Li-COR® Sequencers.

SDS

Catalog Number: (470337-145)

Supplier: 3-D Molecular Designs

Description: Explore biotechnology topics

Supplier: VWR International

Description: AxyPrep™ Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post sequencing reaction

Catalog Number: (470126-538)

Supplier: Edvotek

Description: Learn the basics of DNA sequencing and sequence homology with this lab activity.

Supplier: New England Biolabs (NEB)

Description: This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled).

Certificates

Supplier: AMPLYUS LLC

Description: PCR amplify the ITS region, a fungal DNA barcode that you can sequence and match to genetic databases for molecular identification of fungi.

Supplier: Transgenomic

Description: Transgenomic’s ICEme Kit is an in vitro assay that uses multiplexed PCR (MX PCR) followed by multiplexed ICE COLD-PCR (MX-ICP) for amplification and enrichment of mutations in KRAS Exons 2, 3 and 4, NRAS Exons 2, 3 and 4, BRAF Exons 11 and 15, PIK3CA Exons 9 and 20, EGFR Exons 12, 18, 19, 20 and 21.

Catalog Number: (89139-068)

Supplier: Biotium

Description: PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.

Certificates

Supplier: Quantabio

Description: Superior sensitivity and multiplexing for DNA amplification of long targets.

Catalog Number: (CA10158-728)

Supplier: Cytiva

Description: The AcroPrep™ advance 96-well long tip filter plate for Nucleic Acid Binding (NAB plate) incorporates a silica-based quartz glass fiber media to allow for efficient binding of DNA and RNA, while providing smooth flow and rapid processing of samples.