AxyPrep™ Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post sequencing reaction
- Significant BigDye savings without incurring extra cost and processing time
- High signal to noise ratio resulting in long reads and high QV20+ scores
- Suitable for the clean-up of sequencing reaction of different Sanger sequencing based platforms
- Simple and easy sequencing reaction clean-up process: 25 minutes/96 samples
- No centrifugation or filtration required
Excess dye terminator clean-up post sequencing reaction clean-up is an essential step prior to sequencing. The carry over of excess dye into sequencing results in dye blobs which leads to overwhelming noise and failed calls of sequenced bases, potentially leading to the failure of the sequencing run. AxyPrep™ Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post sequencing reaction in Sanger sequencing. The protocol mainly consists of binding, washing, and elution steps, and can be performed directly in the thermal cycling plate, requiring no centrifugation or filtration.
All spacers and combs are color-coded or labeled to indicate thickness. The 8- and 16-tooth combs are designed for multi-channel pipettor loading.
Each insert has its own electrode assembly that connects to the lid of the buffer tank. Coiled electrodes within the insert generate a high intensity current, allowing standard transfer to take place in approximately one hour. Power leads connect directly to lid so that power is disconnected from system when the lid is removed. Buffer tank is molded to prevent separation and leaking.
Ordering information: System includes buffer tank with power leads and cooling pack; blotting insert; three blotting cassettes; six fiber pads; two 10x10 cm (4x4") notched plates; two 10x10 cm (4x4") plates with spacers; and two 12-tooth, 1mm (1/32") thick combs.
