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Supplier: Spectrum Chemicals
Description: Sodium Phosphate Monobasic, Monohydrate, Granular, BiotechGrade is a reagent with very high buffering capacity widely used in molecular biology, biochemistry and chromatography. Spectrum offers highly pure reagents suitable for biochemical research and analysis that are absent of inhibitors.
Supplier: CUBE BIOTECH
Description: Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Supplier: CUBE BIOTECH
Description: Sulfo-SMA is an electroneutral modification of existing SMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-SMA belongs to a new generation of SMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10.
The special physicochemical properties of Sulfo-SMAs make them ideal for cryo-TEM and other downstream applications.
After successful membrane protein solubilization, the protein can be purified using affinity chromatography. For membrane protein purification, we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Good publications to find details about Sulfo-SMA and Sulfo-DIBMA are:
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: Cytiva
Description: Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
Catalog Number: (CA95017-621L)
Supplier: Cytiva
Description: For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: Cytiva
Description: Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.
Catalog Number: (76371-736)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.

Catalog Number: (76371-738)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.


Catalog Number: (76371-722)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.

SDS


Supplier: VWR International
Description: VWR® brand hematology buffers are designed for use with the range of VWR® hematology stains and reagents.

SDS

Catalog Number: (BDH7830-1)
Supplier: VWR International
Description: N.I.S.T traceable to SRM 919.

Catalog Number: (76371-734)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.

Catalog Number: (76371-740)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.


Catalog Number: (BDH7895-4)
Supplier: VWR International
Description: Made with sodium phosphate monobasic monohydrate, sodium hydroxide solution and deionized water.

Catalog Number: (76371-728)
Supplier: VWR International
Description: VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.


Catalog Number: (CA76463-362)
Supplier: VWR International
Description: VWR® Washing Buffer, 10X, 3D Clear


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