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I-Blue Midi Plasmid Kits (Endotoxin Free), IBI Scientific

Supplier: IBI Scientific
Description: I-Blue Midi Plasmid Endotoxin-Free Kits optimize isolation of plasmid DNA from E.coli with a simple lysis, bind, wash, and elute protocol. The kits include I-Blue lysis buffer, which helps prevent common handling errors, ensuring efficient cell lysis and SDS precipitation.

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SpinOUT™ Protein Desalting and Buffer Exchange Columns, G-Biosciences

Supplier: G-Biosciences
Description: The SpinOUT™ GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of protein solutions ranging from 5µL through to 4mL sample volumes

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I-Blue Mini Plasmid Kits, IBI Scientific

Supplier: IBI Scientific
Description: The I-Blue Mini Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7ml of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization

Product Image-10097-428

Anti-GFP tag Mouse Monoclonal Antibody

Catalog Number: (10097-428)
Supplier: Proteintech
Description: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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Q5 Blood Direct 2X Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Q5 Blood direct 2X master mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood, skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA, K2 EDTA, Na citrate and Na heparin as well samples stored on common preservative filter papers.

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Select-D and NuClean™ Spin Columns, IBI Scientific

Supplier: IBI Scientific
Description: Select-D Columns are ideal for desalting and removing unincorporated radiolabeled deoxynucleotide triphosphates from end labels, fill in, nick-translation, or random primed DNA labeling reactions

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DNA Clean Up, Sephadex® G-50 DNA Grade F, Cytiva

Supplier: Cytiva
Description: Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

SDS Certificates

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Accessories for Calmodulin Resin for CBP Purification, G-Biosciences

Supplier: G-Biosciences
Description: (50mM Tris-HCl pH 7.5 200mM NaCl 2mM EGTA) 100ml

SDS

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MAGicBead™ cfDNA Isolation Kit

Supplier: Zymo Research
Description: Unique, magnetic bead surface chemistry that facilitates unparalleled cfDNA recovery from biofluids.

Product Image-CA95017-597L

MicroSpin Columns, Cytiva

Catalog Number: (CA95017-597L)
Supplier: Cytiva
Description: MicroSpin columns are designed for the rapid purification of DNA in conjunction with Sephadex G-25 DNA Grade for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

Certificates


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ZymoPURE™ 96 Plasmid Miniprep Kits

Supplier: Zymo Research
Description: <p>The ZymoPURE™ 96 plasmid miniprep kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits.</p>

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NEBNext Ultra II FS DNA PCR-free Library Prep Kit with Sample Purification Beads, New England Biolabs

Catalog Number: (CA76538-646)
Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.
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Vivapure® Adenovirus Purification Kits, AdenoPACK, Sartorius

Supplier: Sartorius
Description: The Vivapure® AdenoPACK™ adenovirus purification and concentration kits provides researchers who need to recover up to 3×10<sup>13</sup> purified recombinant adenovirus particles for<i> in vitro</i> transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.

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Glyco-DIBMA in HEPES, PureCube for protein solubilisation

Supplier: CUBE BIOTECH
Description: Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

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E.Z.N.A. DNA/RNA Isolation Kit, Omega Bio-tek

Supplier: Omega Bio-Tek
Description: The E.Z.N.A DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

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pUC19 Vector, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: pUC19 is a commonly used plasmid cloning vector in E.coli

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