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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-CA101640-958

T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Product Image-CA76407-348

Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

Product Image-CA102969-096

NEB PCR Cloning Kit (Without Competent Cells), New England Biolabs

Catalog Number: (CA102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
Product Image-CA102877-584

SPLINTR® Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

Product Image-CA101228-152

Taq DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Product Image-CA102855-130

NEBNEXT® ULTRA™ Ligation Module, New England Biolabs

Catalog Number: (CA102855-130)
Supplier: New England Biolabs (NEB)
Description: The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.
Product Image-CA102500-174

T7 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Product Image-CA102500-168

T3 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

Product Image-CA77025-080

NEBridge Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Product Image-77394-702

Seamless Assembly Master Mix (2X)

Supplier: ReVacc Scientific
Description: This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Product Image-CA102500-054

Gibson Assembly Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Gibson Assembly was developed by Dr

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T4 RNA Ligase 2, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

Product Image-CA101417-940

QUICK BLUNTING™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Product Image-CA102715-904

Thermostable 5' AppDNA/RNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

Product Image-CA102715-912

Gibson Assembly® Cloning Kit, New England Biolabs

Catalog Number: (CA102715-912)
Supplier: New England Biolabs (NEB)
Description: The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.
Product Image-CA101227-656

QUICK LIGATION™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

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