PureCube Co-NTA Agarose XL

Supplier: CUBE BIOTECH

PureCube Co-NTA Agarose XL

PureCube Co-NTA Agarose XL

55403 55405 55410 55412

77393-262EA 358.03 CAD

77393-262 77393-264 77393-266 77393-268

PureCube Co-NTA Agarose XL

Protein Purification Protein Purification Reagents

Our PureCube Co-NTA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used for the purification of active His-tagged proteins from cells.

Usage: Specific binding and purification of 6× His tagged proteins
Specificity: Affinity to His tagged proteins
Binding capacity: ∼30 mg/ml
Bead ligand: Co-NTA
Bead size: 400 μm
Filling quantity: Delivered as a 50% suspension

Our year-long experience in manufacturing agarose resin lead to the high yield of 30 mg protein per ml resin, which is leading in the market compared to other Co-NTA suppliers. PureCube Co-NTA resins are suited for batch spin columns and FPLC. Their huge diameter provides great flow rates for even the most vicious cell media.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

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