PureCube Ni-NTA Agarose XL

Supplier: Cube Biotech

Agarose Purecube Ni NTA XL

Agarose Purecube Ni NTA XL

PureCube

55110 55103 55105 55112

77393-252EA 6050.67 CAD

77393-252 77393-250 77393-254 77393-248

PureCube Ni-NTA Agarose XL

Protein Purification Protein Purification Reagents

Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Usage: Specific binding and purification of 6× His tagged proteins
Specificity: Affinity to His tagged proteins
Binding capacity: 20 to 80 mg/ml, depending on bead size
Bead ligand: Ni-NTA
Bead size: 40 μm, 100 µm, or 400 µm
Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity: Delivered as a 50% suspension

The size of the PureCube Ni-NTA agarose resin ranges from market standard 40 µm and 100 µm diameters to 400 µm (XL). These three options provide plenty of possibilities to choose the correct agarose resin for your purpose. Choose smaller beads for higher mechanical stability and higher protein yield. Meanwhile, bigger beads provide faster flow rates and the possibility of working with very viscous cell media.

Required equipment:
-Lysis Buffer
-Wash Buffer
-Elution Buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents, and equipment Optional: Western Blot reagents and equipment

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