This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
- Origin of MS protein: Human
- Purity: >90% (Determined by SDS-PAGE)
- Number of amino acids: 201 (Human origin), 198 (Mouse origin)
- molecular Mass: 23329 Da (Human origin), 22948 Da (Mouse origin)
- Extinction coefficient (in water) ε280: 25440 M⁻¹cm⁻¹ (Human origin), 20970 M⁻¹cm⁻¹ (Mouse origin)
- Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
- Sequence (Human origin): GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
- Affinity tag: none
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 creates nanodiscs of ∼9 to 10 nm in diameter.
With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs.
Delivery information: Shipping temperature: Room temperature; Storage of lyophilized copolymer: –20 °C for several years; Storage of reconstituted protein: 2 to 8 °C for several day.
