PureCube Ti-NTA MagBeads

Supplier: Cube Biotech
PureCube
31505-TI 31525-TI 31590-TI
77393-600PK 9307.1 CAD
77393-600 77393-576 77393-588
PureCube Ti-NTA MagBeads
Protein Purification Protein Purification Magnetic Beads
PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.

  • Usage: Specific binding and purification of 6× His-tagged proteins, binding of copper-binding proteins
  • Specificity: Affinity to His-tagged proteins, affinity to copper-binding
  • Binding capacity: >80 mg/ml
  • Bead Ligand: Cu-NTA
  • Bead size: 40 μm
  • Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
  • Filling quantity: Delivered as a 50% suspension

Required equipment and materials:
- FPLC hardware like e.g. AKT
- Binding buffer
- Elution buffer

The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Ti-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment with Cube Biotech MagBeads can be automated.
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