HisCube HisCube Ni-Indigo His-Tag Protein Purification MINI Kit

Supplier: CUBE BIOTECH

80101
77393-512EA 1343.63 CAD
77393-512
HisCube HisCube Ni-Indigo His-Tag Protein Purification MINI Kit
Protein Purification Protein Purification Reagents

The HisCube His-tag protein purification MINI kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.


  • Usage: Specific binding and purification of 6× His tagged proteins
  • Specificity: Affinity to His-tagged proteins
  • Binding capacity: >100 mg/ml
  • Bead Ligand: INDIGO-Ni
  • Bead size: 90 μm
  • Filling quantity: Delivered as a 50% suspension
  • pH stability: 2 to 14
  • Chelator stability (e.g. EDTA): 20 mM
  • Reducing agent stability (e.g. DTT): 20 mM
  • Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile


Features - MagBead separator:
Usage: Separation of magnetic beads from the supernatant
Separation of magnetic beads from the supernatant: 1.5 and 2 ml microcentrifuge tubes (Eppis)
Design: Modular (2 microcentrifuges tubes/module): Can be put together onto a larger rack easily
Magnet strength: Above average. To ensure no magnetic beads get lost during the separation process
Area covered on work bench: 3 cm² per module
Cap holder: Included, so that microcentrifuge tubes do not fall close if not wanted
Color: White


The HisCube Ni-INDIGO MINI kit contains our in-house developed PureCube 100 Ni-INDIGO agarose resin and our PureCube 1-Step batch MINI batch spin tubes.


INDIGO's most significant advantage is its tolerance towards reducing and chelating agents. These can be used to great help in His-tag purifications, but traditional His-tag affinity beads do not tolerate them. EDTA is a chelator often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could hinder the access of the purification beads to the His-tagged proteins. However, thanks to INDIGO, both substances can be used now. Therefore, they are part of the kit.

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