PureCube INDIGO Ni-MagBeads

Supplier: Cube Biotech

MagBeads Purecube INDIGO Ni

MagBeads Purecube INDIGO Ni

PureCube

75290 75225

77393-502PK 9725.34 CAD

77393-502 77393-500

PureCube INDIGO Ni-MagBeads

Protein Purification Protein Purification Magnetic Beads

PureCube Ni-INDIGO magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Usage: Specific binding and purification of 6× His tagged proteins
Specificity: Affinity to His-tagged proteins
Binding capacity: >100 mg/ml
Bead ligand: Ni-INDIGO
Bead size: 100 μm
Filling quantity: Delivered as a 50% suspension
pH stability: 2 to 14
Chelator stability (e.g. EDTA): 20 mM
Reducing agent stability (e.g. DTT): 20 mM
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Feature - PureCube mini batch spin column:
Body material: Polypropylene
Filter pore size: 0.1 to 0.2 µm low binding PVDF
Max. volume: 600 µl
Max. g force: 12000 to 14000×g (45-degree fixed angle rotor)
Min. g force: 2500×g for 1 min

Developed with the aim in mind of creating magnetic beads of the highest quality that can tolerate the highest concentrations of chelators like EDTA and DTT, which are often used in mammalian cell culture buffers. The resulting INDIGO ligand has EDTA and DTT stabilities of 20 mM each. That is highly superior to other competing magnetic beads like Ni-NTA.

In biotechnological and pharmaceutical processes in general, the INDIGO-Ni ligand has the advantage over the NTA ligand that no nickel leakage occurs due to its strong binding.

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