Nanodisc Rat MSP1D1-His DMPC

Supplier: Cube Biotech

Nanodisc rat MSP1D1 His DMPC

Nanodisc rat MSP1D1 His DMPC

HiYield-T7

26911

77392-820EA 1249.84 CAD

77392-820

Nanodisc Rat MSP1D1-His DMPC

Protein Expression Protein Expression Reagents

Membrane scaffold protein; rat origin.

Usage: Specific binding and purification of 6× His-tagged proteins
Specificity: Affinity to His-tagged proteins
Binding capacity: >70 mg/ml
Bead ligand: Ni-IDA
Bead size: 30 μm
Filling quantity: Delivered as a 25% suspension
pH stability: 2 to 14
Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 rat creates nanodiscs of ∼9 to 10 nm in diameter.

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein. There are multiple phospholipids to choose from that can make up the body of the nanodisc. Which is best, depends on your protein.

Note that the MSP proteins in these nanodiscs are His-tagged. Keep that in mind when tagging your membrane protein of interest.

Delivery information: Shipping temperature: Dry-Ice; storage of reconstituted protein: −80 °C, avoid freeze-thaw cycles.

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