The Ultrasolute family evolved from the first amphipol A8-35 is used for highly efficient solubilization and stabilization of membrane proteins without the use of detergents while preserving their activity. They demonstrate tolerance to moderate concentrations of divalent cations and exhibit no absorption at wavelengths >250 nm.
- Alternative names: CyclAPol C6-C2-50
- State: Lyophilized powder, to be solved with water
- Buffer: HEPES; pH after solving: 7.5
- Absorbance: <0.1 (280 nm, 1% solution)
- 2-cyclohexyl-ethyl acrylamide/AA Ratio: 1:1
- Solubility: >10% (H₂O)
- Cation tolerance:15 mM Mg²⁺, 10mM Ca²⁺
Ultrasolute™ Amphipol 17 functions like a precision cookie cutter, extracting membrane proteins from the cell membrane while preserving the surrounding native phospholipids. The resulting nanodisc effectively makes the membrane protein into soluble in water, thanks to its hydrophobic surface facing the membrane phospholipids and a hydrophilic surface on the outer side. This distinctive configuration offers a compelling alternative to the conventional detergent-based approach for solubilizing membrane proteins.
Furthermore, Ultrasolute™ Amphipol 17 provides the secondary option of creating nanodiscs using an artificial phospholipid composition, similar to MSP nanodiscs. Demonstrated success has been achieved with phospholipids such as dimyristoyl-glycerol-phosphocholine (DMPC) or palmitoyl-oleoyl-phosphatidyl-choline (POPC). The resulting Ultrasolute™ amphipol 17-nanodisc complex can be utilized in a multitude of biophysical assays, including SDS-PAGE, size exclusion chromatography (SEC), Western Blot, UV/Vis spectroscopy, and various chromatographic procedures, offering versatility and compatibility across a wide range of applications.
To assist researchers in finding the most suitable nanodisc copolymer for their specific membrane protein, Ultrasolute™ amphipol 17 is part of our comprehensive synthetic nanodisc screening kit MAXI, which includes 17 different nanodisc copolymers in total. The product is delivered as a lyophilized powder to be dissolved in ddH₂O. The targeted concentration upon dissolving ranges between 0.1 and 2.5%, with 2.5% being the default concentration.
Delivery information: Shipping temperature: Ambient temperature; storage of lyophilized copolymer: −20 °C for several years; Storage of dissolved copolymer: 2 - 8 °C for several day.
