This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
- Purity: >90% (Determined by SDS-PAGE)
- Number of amino acids: 168
- Molecular Mass: 19488 Da
- Extinction coefficient (in water) ε280: 18450 M⁻¹cm⁻¹
- Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
- Sequence (1-Letter Code), for more details see datasheet: GSTFSKLREQ LGPVTQEFWD NLEKETEGLR QEMSKDLEEV AKVQPYLDDF QKKWQEEMEL RQKVEPLGEE MRDRARAHVD ALRTHLAPYS DELRQRLAAR LEALKENGGA RLAEYHAKAT EHLSTLSEKA KPALEDLRQG LLPVLESFKV SFLSALEEYT KKLNTQ
- Affinity tag: none
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 dH5 creates nanodiscs of ∼7 to 8 nm in diameter.
Delivery information: Shipping Temperature: Dry-Ice. Storage of reconstituted protein: −80 °C, avoid freeze-thaw cycles.