PureCube Co-NTA Agarose

Supplier: Cube Biotech

Agarose Purecube Co NTA

Agarose Purecube Co NTA

PureCube

31403 31415 31405 31412 31401 31410

77392-988PK 16098.24 CAD

77392-988 77392-978 77392-984 77392-986 77392-980 77392-982

PureCube Co-NTA Agarose

Protein Purification Protein Purification Reagents

Our PureCube Co-NTA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.

Usage: Specific binding and purification of 6× His tagged proteins
Specificity: Affinity to His tagged proteins
Binding capacity: >30 mg/ml
Bead Ligand: Co-NTA
Bead size: 40 μm
Filling quantity: Delivered as a 50% suspension
Required equipment:
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 ml tube (min 10000×g)
50 ml centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western -Blot reagents and equipment

Our year-long experience in manufacturing agarose resin led to a high yield of 40 mg protein per ml resin, leading in the market compared to other Co-NTA suppliers. PureCube Co-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability. We recommend our Co-NTA agarose beads with 100 µm diameter for higher flow rates and, for extreme cases, XL-sized Co-NTA beads. Furthermore, these beads are also available pre-packed in a column/cartridge or as magnetic beads.

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