PureCube Compact Cartridge Ni-IDA

Supplier: Cube Biotech

Cartridge compact Ni IDA

Cartridge compact Ni IDA

PureCube

30304 30308 30302 30306

77392-916PK 392.2 CAD

77392-916 77392-918 77392-914 77392-920

PureCube Compact Cartridge Ni-IDA

Protein Purification Protein Purification Reagents

Our PureCube Ni-IDA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They purify active His-tagged proteins from cells and extremely viscous cell media.

Usage: Specific binding and purification of 6× His tagged proteins
Specificity: Affinity to His tagged proteins
Binding capacity: >70 mg/ml
Bead Ligand: Ni-IDA
Bead size: 40 μm
Filling quantity: Delivered as a 50% suspension
pH stability: 2 to 14
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Required equipment and materials:
FPLC hardware like e.g. ÄKTA
Binding Buffer
Elution Buffer

Our year-long experience manufacturing agarose resins led to a high yield of 20 mg protein per ml resin, leading in the market compared to other Ni-IDA suppliers. PureCube Ni-IDA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability. We recommend our Ni-IDA agarose beads with a 40 µm diameter for standard-sized beads with higher protein yield. Furthermore, it is also possible to get the 40 µm Ni-IDA beads pre-packed in a column/cartridge or as magnetic beads. The PureCube Cartridge is compatible with standard FPLC systems like ÄKTA.

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