Amersham ECL Gold Hybridization Buffer, Cytiva

Supplier: Cytiva

ECL detection reagents

Amersham ECL gold hybridization buffer

ECL detection reagents

Amersham ECL gold hybridization buffer

ECL™ ECL™ ECL Direct™

RPN3006 RPN3006

CA95038-280LEA 4005.88 CAD

95038-280 CA95038-280L

Amersham ECL Gold Hybridization Buffer, Cytiva

Electrophoresis Reagents Western Blotting Reagents

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Direct probe labelling in a 10 minute reaction, 1 hour from hybridization to detection with ECL Direct and Hybond™ N+.
Eliminates handling, waste and regulatory issues associated with use of radioactivity.
No need to strip blots before reprobing.
Contains a novel rate enhancer, giving higher sensitivity with very low backgrounds on nylon membranes.

Delivery information: ECL Direct is particularly useful for applications that require a blot to be reprobed several times and also for screening applications requiring rapid throughput and fast results. Each system includes the following reagents, sufficient for labeling 5 to 10 µg nucleic acid and detecting 2000 to 4000 cm ² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

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