Amersham ECL Direct Labeling System, Cytiva

Supplier: Cytiva
ECL™
RPN3005 RPN3005
CA95038-274LEA 1938.9 CAD
CA95038-274L 95038-274
Amersham ECL Direct Labeling System, Cytiva
Electrophoresis Reagents Western Blotting Reagents
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

  • Direct probe labelling in a 10 minute reaction, 1 hour from hybridization to detection with ECL Direct and Hybond™ N+.
  • Eliminates handling, waste and regulatory issues associated with use of radioactivity.
  • No need to strip blots before reprobing.
  • Contains a novel rate enhancer, giving higher sensitivity with very low backgrounds on nylon membranes.

Delivery information: ECL Direct is particularly useful for applications that require a blot to be reprobed several times and also for screening applications requiring rapid throughput and fast results. Each system includes the following reagents, sufficient for labeling 5 to 10 µg nucleic acid and detecting 2000 to 4000 cm ² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.
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