CF® Dye TUNEL Assay Apoptosis Detection Kits, Biotium

Supplier: Biotium

Paraffin section of involuting rat mammary gland stained with CF594 TUNEL assay kit. TUNEL-positive cells are red, nuclei are counterstained blue with DAPI.

Paraffin section of involuting rat mammary gland stained with CF594 TUNEL assay kit. TUNEL-positive cells are red, nuclei are counterstained blue with DAPI.

30063 30064 30074

89410-736EA 1237.9 CAD

89410-736 89410-738 10098-646

CF® Dye TUNEL Assay Apoptosis Detection Kits, Biotium

Assays Nucleic Acid Assays

These kits use enzymatic labeling of DNA double-strand breaks for fluorescence staining of apoptotic cells in fixed samples or tissue sections by TUNEL.

Direct labeling of DNA ds breaks with fluorescent dUTP
Bright & photostable next-generation CF® dyes
For fixed cells or tissue sections
Detect by fluorescence microscopy or flow cytometry

In TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling), TdT enzyme catalyzes the addition of labeled dUTP to the 3'- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kits employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

Kit Components:
Equilibration Buffer
CF® Dye TUNEL Reaction Buffer
TdT Enzyme (calf thymus; recombinant, produced in E. coli)

Delivery information: Kits include reagents for 50 reactions using 50 uL per reaction.

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