MycoAlert™ Mycoplasma Detection Kits, Lonza

Supplier: Lonza
MycoAlert™
LT07-218 LT07-705 LT07-701 LT07-518 LT07-703 LT07-418 LT07-710 LT07-701 LT07-418 LT07-705 LT07-703 LT07-118 LT07-518 LT07-710 LT07-318 LT07-218 LT07-118 LT07-318
CA10064-052EA 1536.55 CAD
75866-212 CA10064-052 CA10064-054 CA11006-552 CA11006-554 CA11006-550 CA11006-548 75860-358 CA11006-556 75870-452 75870-450 75860-362 75870-456 75870-454 75860-360 CA10064-050 75870-458 CA10063-952
MycoAlert™ Mycoplasma Detection Kits, Lonza
Assays Enzyme Assays
The MycoAlert™ PLUS Mycoplasma Detection Kit is the next generation Lonza MycoAlert™ Kit. It provides higher light output to allow for use with less sensitive plate luminometers or multifunctional readers and testing of unused media, media supplements or water.

  • Convenient & fast
  • Simply add two reagents to the culture supernatant, perform two luminescence readings and get your results in less than 20 minutes
  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • Suited for tube luminometers, plate luminometers and multifunctional readers

The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells

MycoAlert™ Assay Control Set (available separately)
The MycoAlert™ Assay Control Set includes a lyophilized positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ PLUS Mycoplasma Detection Kit. It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio of <1.

Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
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